set.seed(123)
small_mat <- matrix(rnorm(56), nrow = 8)
rownames(small_mat) <- paste0("row", seq_len(nrow(small_mat)))
colnames(small_mat) <- paste0("column", seq_len(ncol(small_mat)))Operators
Addition operator
+: adds elements to the active plot in the active layout.
The + operator is straightforward and should be used as
needed.
In quad_layout()
(ggheatmap()/ggside()), four nested layouts
are available for annotations: top, left,
bottom, and right. If no active annotation is
set, quad_layout()
(ggheatmap()/ggside()) treat itself as the
active layout. Since there is only one main plot in the
quad_layout()
(ggheatmap()/ggside()), the main plot is
always treated as the active plot in this context.
# Initialize the heatmap; by default, no active annotation will be set.
# The active layout is the heatmap layout, and the active plot in the layout is
# the main plot.
ggheatmap(small_mat) +
# Add elements to the main plot
scale_fill_viridis_c() +
# Change the active layout to the left annotation
anno_left(size = 0.2) +
# Add a dendrogram in the left annotation
align_dendro() +
# Change the active layout to the right annotation
anno_right(size = 0.2) +
# Add a dendrogram in the right annotation
align_dendro()
#> → heatmap built with `geom_tile()`
In ggstack()/stack_layout(), a nested
layout will only occur if you pass a quad_layout() into
ggstack()/stack_layout(). If the active plot
within the ggstack()/stack_layout() is
quad_layout(), this quad_layout() is treated
as the active layout, and the + operator will add elements
to it, following the same principles as in quad_layout().
Otherwise, the ggstack()/stack_layout() itself
is treated as the active layout, and the + operator will
add elements directly to this plot.
stack_alignh(small_mat) +
# the dendrogram will be added to the stack
align_dendro() +
# Add elements to the dendrogram
geom_point() +
# add a heamtap layout to the stack
ggheatmap() +
# the active layout is the heamtap layout
# so following elements will be added to the heatmap layout
theme(axis.text.x = element_text(angle = -60, hjust = 0)) +
anno_right() +
align_dendro()
#> → heatmap built with `geom_tile()`
logical AND operator
&: applies elements to all plots in the layout.
The & operator works similarly to
patchwork, applying an element across all plots in a
layout. Since & has lower precedence than
+, it’s generally best to use it at the end of an
expression or you should wrap it in parentheses when needed.
# Initialize the heatmap with color scales and annotations.
ggheatmap(small_mat) +
scale_fill_viridis_c() +
anno_left(size = 0.2) +
# Add a dendrogram in the left annotation and split the dendrogram into 3 groups
align_dendro(aes(color = branch), k = 3L) +
anno_right(size = 0.2) +
# Add a dendrogram in the right annotation and split the dendrogram into 3 groups
align_dendro(aes(color = branch), k = 3L) &
# Set color scale for all plots
scale_color_brewer(palette = "Dark2")
#> → heatmap built with `geom_tile()`
Subtraction operator
The - operator is more powerful than the +
operator, enabling flexible addition of elements to multiple plots.
While its use might initially seem unintuitive, the reason behind this
is that - shares the same precedence group as
+, which allows it to seamlessly combine with
+.
quad_layout()
If the active layout is the
ggheatmap()/quad_layout() itself, the
- operator behaves similarly to &. It
applies the specified elements to all plots within the layout.
# Initialize the heatmap
ggheatmap(small_mat) +
scale_fill_viridis_c() +
anno_left(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) +
anno_right(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) +
# Remove any active annotation
quad_active() -
# Set color scale for all plots, since the active layout is the `ggheatmap()`/`quad_layout()`
scale_color_brewer(palette = "Dark2")
#> → heatmap built with `geom_tile()`
If the active layout is the annotation stack, the -
operator will only add the elements to all plots in the active
annotation stack:
ggheatmap(small_mat) +
scale_fill_viridis_c() +
anno_left(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) +
align_dendro(aes(color = branch), k = 3L) -
# Modify the the color scales of all plots in the left annotation
scale_color_brewer(palette = "Dark2")
#> → heatmap built with `geom_tile()`
stack_layout()
If the active layout is the
ggstack()/stack_layout() itself,
- applies the elements to all plots in the layout except
the nested ggheatmap()/quad_layout().
stack_alignv(small_mat) +
align_dendro() +
ggtitle("I'm from the parent stack") +
ggheatmap() +
# remove any active context
stack_active() +
align_dendro() +
ggtitle("I'm from the parent stack") -
# Modify the the color scales of all plots in the stack layout except the heatmap layout
scale_color_brewer(palette = "Dark2") -
# set the background of all plots in the stack layout except the heatmap layout
theme(plot.background = element_rect(fill = "red"))
#> → heatmap built with `geom_tile()`
When the active layout is the nested
ggheatmap()/quad_layout(), the -
operator applies the elements to this nested layout, following the same
principles as in the
ggheatmap()/quad_layout().
Want apply elements for plots in both stack_layout() and
the nested ggheatmap()/quad_layout() at the
same time? refer to the following section.
with_quad
Along with the - operator, with_quad() can
be used to modify the operated context of
ggheatmap()/quad_layout().
This function accepts three arguments:
-
x: The object to be applied using the
-operator. -
position: A string containing one or more of
"t","l","b", and"r"specifies the context for applyingx. -
main: A single boolean value indicates whether
xshould also apply to the main plot withinggheatmap()/quad_layout(). Only used whenpositionis notNULL.
quad_layout()
By default, if the active layout is the
ggheatmap()/quad_layout() itself,
with_quad() won’t do something special. But when the active
context in ggheatmap()/quad_layout() is set to
top or bottom, by wrapping object with
with_quad(), the - operator will apply changes
not only to that annotation but also to the opposite one (i.e., bottom
if top is active, and vice versa). The same principle applies to the
left and right annotation. In these cases, the object will also be
applied to the main plot by default unless specified by
main argument otherwise.
ggheatmap(small_mat) +
scale_fill_viridis_c() +
anno_left(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) +
# Change the active layout to the left annotation
anno_top(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) +
anno_bottom(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) -
# Modify the color scale of all plots in the bottom and the opposite annotation
# in this way, the `main` argument by default would be `TRUE`
with_quad(scale_color_brewer(palette = "Dark2", name = "Top and bottom"))
#> → heatmap built with `geom_tile()`
The position argument can be a string containing one or
more of "t", "l", "b", and
"r", indicating which annotation stack should be used as
the context. When the position argument is manually set,
the default value of the main argument will be
FALSE.
ggheatmap(small_mat) +
scale_fill_viridis_c() +
anno_left(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) +
anno_top(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) +
anno_bottom(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) -
# Modify the background of all plots in the left and top annotation
with_quad(theme(plot.background = element_rect(fill = "red")), "tl")
#> → heatmap built with `geom_tile()`
Setting position to NULL removes any active
annotation stack, meaning the object will apply to all plots in the
layout, including annotation stacks:
ggheatmap(small_mat) +
scale_fill_viridis_c() +
anno_left(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) +
anno_top(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) +
anno_bottom(size = 0.2) +
align_dendro(aes(color = branch), k = 3L) -
# Modify the background of all plots
with_quad(theme(plot.background = element_rect(fill = "red")), NULL)
#> → heatmap built with `geom_tile()`
stack_layout()
By default, when the active layout is the stack_layout()
itself, by wrapping object with with_quad(), -
operator will apply changes to all plots along the
stack_layout(), which means if the stack layout is in
horizontal, - operator will also add the element to the
left and right annotation, if the stack layout is in vertical,
- operator will also add element to the top and bottom
annotation. In these cases, the object will also be applied to the main
plot by default unless specified by main argument
otherwise.
stack_alignv(small_mat) +
align_dendro() +
ggtitle("I'm from the parent stack") +
ggheatmap() +
anno_top() +
align_dendro() +
ggtitle("I'm from the nested heatmap") +
# remove any active context
stack_active() +
align_dendro() +
ggtitle("I'm from the parent stack") -
# Modify the the color scales of all plots in the stack layout except the heatmap layout
scale_color_brewer(palette = "Dark2") -
# set the background of all plots in the stack layout (including plots in the heatmap layout)
with_quad(theme(plot.background = element_rect(fill = "red")))
#> → heatmap built with `geom_tile()`
When the active layout is the nested
ggheatmap()/quad_layout(), the -
operator applies the elements to this nested layout, following the same
principles as for
ggheatmap()/quad_layout().
Session information
sessionInfo()
#> R version 4.4.2 (2024-10-31)
#> Platform: x86_64-pc-linux-gnu
#> Running under: Ubuntu 22.04.5 LTS
#>
#> Matrix products: default
#> BLAS: /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3
#> LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.20.so; LAPACK version 3.10.0
#>
#> locale:
#> [1] LC_CTYPE=C.UTF-8 LC_NUMERIC=C LC_TIME=C.UTF-8
#> [4] LC_COLLATE=C.UTF-8 LC_MONETARY=C.UTF-8 LC_MESSAGES=C.UTF-8
#> [7] LC_PAPER=C.UTF-8 LC_NAME=C LC_ADDRESS=C
#> [10] LC_TELEPHONE=C LC_MEASUREMENT=C.UTF-8 LC_IDENTIFICATION=C
#>
#> time zone: UTC
#> tzcode source: system (glibc)
#>
#> attached base packages:
#> [1] stats graphics grDevices utils datasets methods base
#>
#> other attached packages:
#> [1] ggalign_0.0.5 ggplot2_3.5.1
#>
#> loaded via a namespace (and not attached):
#> [1] gtable_0.3.6 jsonlite_1.8.9 dplyr_1.1.4 compiler_4.4.2
#> [5] tidyselect_1.2.1 jquerylib_0.1.4 systemfonts_1.1.0 scales_1.3.0
#> [9] textshaping_0.4.0 yaml_2.3.10 fastmap_1.2.0 R6_2.5.1
#> [13] labeling_0.4.3 generics_0.1.3 knitr_1.49 tibble_3.2.1
#> [17] desc_1.4.3 munsell_0.5.1 RColorBrewer_1.1-3 bslib_0.8.0
#> [21] pillar_1.9.0 rlang_1.1.4 utf8_1.2.4 cachem_1.1.0
#> [25] xfun_0.49 fs_1.6.5 sass_0.4.9 viridisLite_0.4.2
#> [29] cli_3.6.3 pkgdown_2.1.1 withr_3.0.2 magrittr_2.0.3
#> [33] digest_0.6.37 grid_4.4.2 lifecycle_1.0.4 vctrs_0.6.5
#> [37] evaluate_1.0.1 glue_1.8.0 farver_2.1.2 ragg_1.3.3
#> [41] fansi_1.0.6 colorspace_2.1-1 rmarkdown_2.29 tools_4.4.2
#> [45] pkgconfig_2.0.3 htmltools_0.5.8.1